Goat Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – HRP

Purified Rabbit IgG (H&L) (>98%)

This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

This HRPO conjugate is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

1:500 - 1:5,000 for immunohistochemistry on tissue sections 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates

Goat Anti-Rabbit IgG (H&L) recognizes Rabbit IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

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2.) Mesa RA and Roberson EDO. (2020) F1000Research. 8:1211 Journal Link
3.) Low, J. et al. (2020) Cell Reports 33(9): 108450 Journal Link
4.) Mesa, R. et al. (2019) F1000Research 8(1211):10.12688/f1000research.19803.2 Journal Link