Anti-Human BMI-1 (Intermediate Domain)

PN:B501

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human BMI-1 recognizes an epitope in the intermediate domain of human, mouse and rat BMI-1. This polyclonal antibody was purified using affinity chromatography.

The transcriptional repressor BMI-1 was first identified as a proto-oncogene frequently activated by Moloney murine leukemia proviral insertions in mice and cooperating with c-myc in the generation of mouse lymphomas. BMI-1 is involved in segment specification, cell growth and maintenance, transcriptional regulation, and chromatin modification. A major target of BMI-1 is the ink4a locus which encodes tumor suppressor proteins p16 and p19Arf, which are important in tumor progression and thought to be critical in cell proliferation and senescence.² Recent studies have also shown that BMI-1 is required for the maintenance of adult normal and leukemic stem cells,³ suggesting that BMI-1 could an attractive therapeutic target for stem cell proliferation and renewal as well as for anti-cancer strategies.

1. Alkema, MJ. et al. (1993) Hum. Mol. Genet. 2:1597
2. Jacobs, J J. et al. (1999) Nature 397:164
3. Lessard, J. et al. (2003) Nature 255