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Recombinant Human Angiotensin-Converting Enzyme 2 (ACE-2)
Purified No Carrier Protein
(ACEH)
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Prod. No.:A352
SourceNSO Cells
Pkg. Size:10 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Angiotensin I converting enzyme 2 (ACE2) is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin[1-9].1 ,or the conversion of angiotensin II to angiotensin 1-7.2 ACE 2 has direct effects on cardiac function2, and is expressed predominantly in vascular endothelial cells of the heart and the kidneys.3 ACE2 plays a regulatory role in lung pathophysiology, including pulmonary fibrosis and acute lung disease. Activation of ACE2 by a small molecule can be a therapeutically relevant approach for treating and controlling Pulmonary Hypertension (PH).4 ACE2 may also have clinical potential as a novel molecular target for the treatment of pancreatic ductal adenocarcinoma (PDAC).5

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human ACE-2 is Mr 85 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 120 kDa.
State of Matter
Solution
Formulation
This recombinant protein was 0.2 µm filtered and is supplied in a solution of sodium chloride (NaCl), zinc chloride (ZnCl2), tris and glycerol.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
Working aliquots of this recombinant protein solution are stable for up to six months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at -20° to -70°C for three months without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
qst ieeqaktfld kfnheaedlf yqsslaswny ntniteenvq nmnnagdkws aflkeqstla qmyplqeiqn ltvklqlqal qqngssvlse dkskrlntil ntmstiystg kvcnpdnpqe clllepglne imansldyne rlwaweswrs evgkqlrply eeyvvlknem aranhyedyg dywrgdyevn gvdgydysrg qliedvehtf eeikplyehl hayvraklmn aypsyispig clpahllgdm wgrfwtnlys ltvpfgqkpn idvtdamvdq awdaqrifke aekffvsvgl pnmtqgfwen smltdpgnvq kavchptawd lgkgdfrilm ctkvtmddfl tahhemghiq ydmayaaqpf llrnganegf heavgeimsl saatpkhlks igllspdfqe dneteinfll kqaltivgtl pftymlekwr wmvfkgeipk dqwmkkwwem kreivgvvep vphdetycdp aslfhvsndy sfiryytrtl yqfqfqealc qaakhegplh kcdisnstea gqklfnmlrl gksepwtlal envvgaknmn vrpllnyfep lftwlkdqnk nsfvgwstdw spyadqsikv rislksalgd kayewndnem ylfrssvaya mrqyflkvkn qmilfgeedv rvanlkpris fnffvtapkn vsdiiprtev ekairmsrsr indafrlndn sleflgiqpt lgppnqppvs hhhhhhhhhh



Country of Origin
USA
References
1. Acton S et al. (2000) Circ Res. 87: E1
2. Gamliel-Lazarovich A et al. (2007) Cardiovasc Res. 73: 463
3. Nabel EG et al. (2000) N Engl J Med. 347: 1795
4. Raizada MK et al. (2009) Am J Respir Crit Care Med.
5. Yuan Y et al. (2009) Tohoku J Exp Med. 217: 123
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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