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Recombinant Mouse Persephin (PSP)
Purified No Carrier Protein
(PSPN)
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Prod. No.:P233
SourceE. coli
Pkg. Size:5 µg, 20 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Persephin (PSP) is a secreted protein belonging to the glial cell line-derived neurotrophic factor (GDNF) family of the TGF-b superfamily. Just like factor GDNF and neurturin (NTN), PSP promotes the survival of ventral midbrain dopaminergic neurons and prevents their degeneration. Persephin also supports the survival of motor neurons.1 Persephin is expressed at very low levels in most tissues. It is synthesized throughout the nervous system and has both astroglial and neuronal origin.2

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse PSP is Mr 10 kDa.
State of Matter
Lyophilized
Purity
>98% by SDS Page and HPLC
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse PSP was determined by its ability to stimulate proliferation of TT cells. The expected ED50 for this effect is typically 1 - 3 ng/mL.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
The DNA sequence encoding the TGF-β-like domain of mouse Persephin (Ala 61 - Gly 156) was inserted into a suitable expression vector and was expressed in E. coli (Tomac, A.C. et al., 2002, Proc. Natl. Acad. Sci. USA 99:9521 - 6526).



Country of Origin
USA
References
1. Johnson EM et al. (1998) Neuron 20: 245
2. Krieglstein K. et al. (1998) J Neurosci Res. 53: 494
Related Products
Recombinant Human Persephin (Leinco Prod. No.: P136)
Anti-Human Persephin (Antigen Aff. Pur.) (Leinco Prod. No.: P172)
Anti-Human Persephin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: P173)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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