Product Datasheet
www.leinco.com
excellence in early discovery research       



Anti-Human Bone Morphogenetic Protein-2 (BMP-2)
Purified No Carrier Protein
(BMP-2A)
Monoclonal Antibody
Share |


Prod. No.:B649
Clone:100221
Isotype:Mouse IgG2b
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human Bone morphogenetic protein 2 (BMP-2) (Clone 100221) recognizes an epitope on Human BMP-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human BMP-2 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml.
Western Blotting: To detect Human BMP-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human BMP-2 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human BMP-2 (Leinco Prod. No.: B388).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human BMP-2 (Leinco Prod. No.: B388)
Anti-Human BMP-2 (Antigen Aff. Pur.) (Leinco Prod. No.: B420)
Anti-Human BMP-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B421)
Anti-Human BMP-2 (Leinco Prod. No.: B576)
Anti-BMP-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B610)
Human BMP-2 ELISA Development Kit (Leinco Prod. No.: B422)
Recombinant Human BMP-2 (Leinco Prod. No.: B625)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
359 Consort Drive | St. Louis, MO 63011 | (800) 538-1145 | (636) 527-5545 fax
800.538.1145 ® Copyright Leinco Technologies, Inc. 2006 – 2012