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Anti-Human Desmoglein-2 (DSG2)
Purified No Carrier Protein
(ARVC10, ARVD10, CDHF5, HDGC, MGC117034, MGC117036, MGC117037)
Monoclonal Antibody
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Prod. No.:D356
Clone:141409
Isotype:Mouse IgG1
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human Desmoglein-2 (DSG2) (Clone 141409) recognizes an epitope on Human DSG2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Desmoglein 2, also known as DSG2, is a human gene. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It provides cellular adhesion and forces transduction by cell-to-cell anchorage. 1 It also plays a role in embryonic stem cell proliferation.

Host Species
Mouse
Immunogen
NS0-Derived Recombinant Human Desmoglein-2
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human Desmoglein-2 is approximately 0.5 ng/well.
Western Blotting: To detect Human Desmoglein-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human Desmoglein-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Mol Genet Metab. et al.(2008) Epub 95: 74
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human Desmoglein-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: D345)
Anti-Human Desmoglein-2 (Antigen Aff. Pur.) (Leinco Prod. No.: D350)
Recombinant Human Desmoglein-2 (Leinco Prod. No.: D340)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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