Anti-Human Desmoglein-2 (Clone 141409)

Anti-Human Desmoglein-2 (Clone 141409)

Product No.: D356

[product_table name="All Top" skus="D356"]

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Clone
141409
Target
Desmoglein-2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
ARVC10, ARVD10, CDHF5, HDGC, MGC117034, MGC117036, MGC117037
Isotype
IgG1
Applications
ELISA Indirect
,
ICC
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
NS0-Derived Recombinant Human Desmoglein-2 (Accession # CAA81226)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human Desmoglein-2 is approximately 0.5 ng/well.
Western Blotting: To detect Human Desmoglein-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Desmoglein-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
Immunocytochemistry: Suitable for use at concentration of 5-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Desmoglein-2 (DSG2) (Clone 141409) recognizes an epitope on Human DSG2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Desmoglein 2, also known as DSG2, is a human gene. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It provides cellular adhesion and forces transduction by cell-to-cell anchorage. 1 It also plays a role in embryonic stem cell proliferation.

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Ozcelik, C. et al. (2008) Mol Genet Metab. 95(1-2):74-80.
Indirect Elisa Protocol
ICC
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.