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Goat Anti-Mouse Activin Receptor-Like Kinase 1 (ALK-1)
Antigen Affinity Purified
(ACVRL1, ACVRLK1, HHT, HHT2, ORW2, SKR3, TSR-I)
Polyclonal Antibody
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Prod. No.:A390
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Mouse Activin-Receptor-Like Kinase-1 (ALK-1) recognizes Mouse ALK-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Activin A receptor type II-like 1, also known as ACVRL1, is a human gene. ACVRL1 is a receptor in the TGF beta signaling pathway. It is also known as "activin receptor-like kinase 1", or "ALK1". This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 suggesting a critical role for ALK-1 in the control of blood vessel development or repair.

Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse ALK-1 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/ml. A suitable detection antibody is Anti-Mouse -ALK-1Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: A404) at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is Recombinant Mouse ALK-1 (Leinco Prod. No.: A349).
Western Blotting: To detect Mouse ALK-1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse ALK-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse ALK-1 (Leinco Prod. No.: A349).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Anti-Mouse -ALK-1Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: A404)
Recombinant Mouse ALK-1 (Leinco Prod. No.: A349)
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human ALK-1 (Leinco Prod. No.: A343)
Anti-Human ALK-1 (Antigen Aff. Pur.) (Leinco Prod. No.: A367)
Anti-Human ALK-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: A398)
Anti-Human ALK-1 (Leinco Prod. No.: A378)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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