Anti-Human ALK-1 (Clone 117720)

Pricing & Details

Product No.A378
Clone
117720
Protein
ALK-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Activin Receptor-Like Kinase 1, ACVRL1, ACVRLK1, HHT, HHT2, ORW2, SKR3, TSR-I
Isotype
Mouse
IgG2a
Applications
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
A378-25 µg
25 µg
$175.00
In stock
Max:
Min: 1
Step: 1
A378-100 µg
100 µg
$359.00
In stock
Max:
Min: 1
Step: 1
A378-500 µg
500 µg
$799.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human ALK-1 (Accession # P37023)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human ALK-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human ALK-1 is 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:A343.
Simple Western: Suitable for use at concentration of 40 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Activin Receptor-Like Kinase 1 (ALK-1) (Clone 117720) recognizes an epitope on Human ALK-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 1% cross-reactivity with rmALK-1, rhActivin RIA, and rhActivin RIB.
Antigen Distribution
ALK-1 is expressed in endothelial cells and other highly vascularized tissues
Background
Activin A receptor type II-like 1, also known as ACVRL1, is a human gene. ACVRL1 is a receptor in the TGF beta signaling pathway. It is also known as "activin receptor-like kinase 1", or "ALK1". This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 suggesting a critical role for ALK-1 in the control of blood vessel development or repair.

Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

Antigen Details

Protein
PubMed
General Western Blot Protocol

Formats Available

Prod No.
Description
A378
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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