Product Datasheet
www.leinco.com
excellence in early discovery research       



Goat Anti-Human Insulin-Like Growth Factor I (IGF-I)
Antigen Affinity Purified
(Somatamedin C, IGF-IA )
Polyclonal Antibody
Share |


Prod. No.:I-142
Host:Goat
Pkg. Size:250 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human Insulin Like Growth Factor-I (IGF-1) recognizes Human IGF-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Production of IGF-1is stimulated by growth hormone. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. In addition to the insulin-like effects, IGF-1 can also regulate cell growth and development, especially in nerve cells, as well as cellular DNA synthesis. IGF-1 is closely related to a second protein called IGF-2. IGF-1 is used by physicians as a screening test for growth hormone deficiency and excess. The IGF signaling pathway appears to play a crucial role in cancer. Several studies have shown that increased levels of IGF lead to an increased risk of cancer.1

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human IGF-1 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.25 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Govindan R et al. (2006) Journal of thoracic oncology 1: 607
Related Products
Recombinant Mouse IGF-I (Leinco Prod. No.: I-239)
PN:
Anti-Mouse IGF-I (Antigen Aff. Pur.) (Leinco Prod. No.: I-340)
Anti-Human IGF-I-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-235)
Anti-Mouse IGF-I-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-240)
Anti-Human IGF-I (Leinco Prod. No.: I-649)
Anti-Mouse IGF-I (Leinco Prod. No.: I-709)
Recombinant Human IGF-I R (Leinco Prod. No.: I-841)
Recombinant Rat IGF-I (Leinco Prod. No.: I-524)
Recombinant Human IGF-I (Leinco Prod. No.: I-180)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
359 Consort Drive | St. Louis, MO 63011 | (800) 538-1145 | (636) 527-5545 fax
800.538.1145 ® Copyright Leinco Technologies, Inc. 2006 – 2013