Anti-Human IGF-I

Anti-Human IGF-I

Product No.: I-142

[product_table name="All Top" skus="I-142"]

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Target
IGF-I
Product Type
Polyclonal Antibody
Alternate Names
Insulin-Like Growth Factor I, Somatamedin C, IGF-IA
Applications
ICC
,
IHC FFPE
,
N
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human IGF-1 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Additional Applications Reported In Literature ?
Immunocytochemistry: Suitable at concentration of 5-15 µg/mL.
Immunohistochemistry: Suitable at concentration of 5-15 µg/mL.
Neutralization: Measured by its ability to neutralize IGF-I/IGF-1-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 6 ng/mL Recombinant Human IGF-I/IGF-1.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Insulin Like Growth Factor-I (IGF-1) recognizes Human IGF-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Production of IGF-1is stimulated by growth hormone. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. In addition to the insulin-like effects, IGF-1 can also regulate cell growth and development, especially in nerve cells, as well as cellular DNA synthesis. IGF-1 is closely related to a second protein called IGF-2. IGF-1 is used by physicians as a screening test for growth hormone deficiency and excess. The IGF signaling pathway appears to play a crucial role in cancer. Several studies have shown that increased levels of IGF lead to an increased risk of cancer.1

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Govindan, R. et al. (2006) Journal of thoracic oncology 1: 607
ICC
IHC FFPE
N
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.