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Recombinant Human Betacellulin (BTC)
Purified No Carrier Protein
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Prod. No.:B268
SourceE. coli
Pkg. Size:10 µg, 50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Betacellulin, also known as BTC, is a member of the EGF family of cytokines. BTC is initially synthesized as a glycosylated transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence (1). Several tissues including kidney, liver, pancreas, uterus, small intestine as well as certain tumor cells express BTC (2). In addition, it can also be found in bodily fluids including serum, milk and colostrum (3). BTC is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (4). It also promotes pancreatic beta-cell growth and differentiation (5). The primary receptor for BTC is the receptor for EGF, specifically ErbB-1 and ErbB-4 (6).

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human BTC is Mr 9 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Betacellulin is determined by the dose-dependent stimulation of the proliferation of mouse Balb/3T3 cells. The expected ED50 for this effect is 0.15 - .06 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
dgnstrspet ngllcgdpee ncaatttqsk rkghfsrcpk qykhycikgr crfvvaeqtp scvcdegyig arcervdlfy



Country of Origin
USA
References
1. Tada, H. et al. (1999) J. Cell Biochem. 72:423
2. Shing, Y. et al. (1993) Science 259:1604
3. Bastian, S. E. et al. (2001) J. Endocrnol. 168:203
4. Dunbar, A. J. et al. (2000) J. Biochem. Cell Bio. 32:805
5. Li, L. et al. (2001) Endocrinology 142:5379
6. Watanabe, T. et al. (1994) J. Biol. Chem. 269:9966
Related Products
Recombinant Mouse Betacellulin (Leinco Prod. No.: B527)
Human Betacellulin ELISA Development Kit (Leinco Prod. No.: B620)
Anti-Human Betacellulin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B609)
Anti-Human Betacellulin (Leinco Prod. No.: B572)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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