Recombinant Human Betacellulin

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Pricing & Details

B268
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
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B268-10 µg
10 µg
$299.00
In stock
Max:
Min: 1
Step: 1
B268-50 µg
50 µg
$915.00
In stock
Max:
Min: 1
Step: 1
B268-250 µg
250 µg
$1,849.00
In stock
Max:
Min: 1
Step: 1
B268-1000 µg
1000 µg
$5,200.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Background

Betacellulin, also known as BTC, is a member of the EGF family of cytokines. BTC is initially synthesized as a glycosylated transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence (1). Several tissues including kidney, liver, pancreas, uterus, small intestine as well as certain tumor cells express BTC (2). In addition, it can also be found in bodily fluids including serum, milk and colostrum (3). BTC is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (4). It also promotes pancreatic beta-cell growth and differentiation (5). The primary receptor for BTC is the receptor for EGF, specifically ErbB-1 and ErbB-4 (6).

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Betacellulin is determined by the dose-dependent stimulation of the proliferation of mouse Balb/3T3 cells. The expected ED50 for this effect is 0.15 - .06 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
dgnstrspet ngllcgdpee ncaatttqsk rkghfsrcpk qykhycikgr crfvvaeqtp scvcdegyig arcervdlfy
N-terminal Sequence Analysis
Asp32
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human Betacellulin is 9 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 15 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Tada, H. et al. (1999) J. Cell Biochem. 72:423
2. Shing, Y. et al. (1993) Science 259:1604
3. Bastian, SE. et al. (2001) J. Endocrnol. 168:203
4. Dunbar, A. J. et al. (2000) J. Biochem. Cell Bio. 32:805
5. Li, L. et al. (2001) Endocrinology 142:5379
6. Watanabe, T. et al. (1994) J. Biol. Chem. 269:9966
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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