Recombinant Human Betacellulin

Betacellulin, also known as BTC, is a member of the EGF family of cytokines. BTC is initially synthesized as a glycosylated transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence (1). Several tissues including kidney, liver, pancreas, uterus, small intestine as well as certain tumor cells express BTC (2). In addition, it can also be found in bodily fluids including serum, milk and colostrum (3). BTC is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (4). It also promotes pancreatic beta-cell growth and differentiation (5). The primary receptor for BTC is the receptor for EGF, specifically ErbB-1 and ErbB-4 (6).

The biological activity of Human Betacellulin is determined by the dose-dependent stimulation of the proliferation of mouse Balb/3T3 cells. The expected ED₅₀ for this effect is 0.15 - .06 ng/ml.

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Asp32

Lyophilized

The predicted molecular weight of Recombinant Human Betacellulin is 9 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 15 kDa.

9

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Tada, H. et al. (1999) J. Cell Biochem. 72:423
2. Shing, Y. et al. (1993) Science 259:1604
3. Bastian, SE. et al. (2001) J. Endocrnol. 168:203
4. Dunbar, A. J. et al. (2000) J. Biochem. Cell Bio. 32:805
5. Li, L. et al. (2001) Endocrinology 142:5379
6. Watanabe, T. et al. (1994) J. Biol. Chem. 269:9966