Product Datasheet
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Recombinant Human BimL (BimL)
Purified No Carrier Protein
(Bcl2-L-11, BAM, BimL, BCL2-like 11, BOD, Bim)
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Prod. No.:B621
SourceE. coli
Pkg. Size:50 µg, 1 mg
Storage:-20 to -70ºC Detailed storage instructions below.
Description
Background:
BimL a pro-apoptotic member of the BCL-2 protein family is also called Bod, BAM, or B2L11. This protein is one of several splice variants and interacts with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and act as an apoptotic activator. Its expression can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role in neuronal and lymphocyte apoptosis. Transgenic studies in the mouse suggested that this protein functions as an essential initiator of the apoptosis in thymocyte-negative selection by binding and inhibiting the activity of anti-apoptoticBcl-2 family members, thereby inducing the release of cytochrome c from mitochondria.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human BimL is Mr 14.3 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 16 kDa.
State of Matter
Solution
Formulation
This recombinant protein was 0.2µm filtered and is supplied in a solution of 20mM HEPES-KOH, 1mM DTT, 100mM KCL and 2% Glycerol at a pH of 8.0.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic opening, this protein may be stored at 2°C to 8°C for (1) one week. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Bim was determined by its ability to neutralize anti-apoptotic Bcl-2 and quantified using a functional ELISA. The expected ED50=0.1 - 2.0µM.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
akqpsdvsse cdregrqlqp aerppqlrpg aptslqtepq drspapmscd kstqtpsppc qafnhylsam asmrqaepad mrpeiwiaqe lrrigdefna yyarrvflnn yqaaedhpr (hhhhhh)



Country of Origin
USA
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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