Recombinant Human Bim L

BimL a pro-apoptotic member of the BCL-2 protein family is also called Bod, BAM, or B2L11. This protein is one of several splice variants and interacts with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and act as an apoptotic activator. Its expression can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role in neuronal and lymphocyte apoptosis. Transgenic studies in the mouse suggested that this protein functions as an essential initiator of the apoptosis in thymocyte-negative selection by binding and inhibiting the activity of anti-apoptoticBcl-2 family members, thereby inducing the release of cytochrome c from mitochondria.

The biological activity of Human Bim was determined by its ability to neutralize anti-apoptotic Bcl-2 and quantified using a functional ELISA. The expected ED₅₀=0.1 - 2.0µM.

Histidine Tag

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His

Solution

The predicted molecular weight of Recombinant Human Bim_L is Mr 14.3 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 16 kDa.

14.3

This recombinant protein was 0.2µm filtered and is supplied in a solution of 20mM HEPES-KOH, 1mM DTT, 100mM KCL and 2% Glycerol at a pH of 8.0.

This protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic opening, this protein may be stored at 2°C to 8°C for (1) one week. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Dry Ice