NSO Cells

The predicted molecular weight of Recombinant Human Desmoglein-2 is Mr 89 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 115 kDa.

Lyophilized

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

>95% by SDS-PAGE and analyzed by silver stain.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

<1.0 EU/µg as determined by the LAL method

The biological activity of Human DSG2 was determined by the ability of the immobilized protein to support the adhesion of BUD8 human skin fibroblast cells. When 3 x 10⁴ cells are added to recombinant human Desmoglein 2 coated plates (10 μg/ml, 100 μl/well), approximately 50%-80% will adhere after 1 hour at 37°C.

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USA

Anti-Human Desmoglein-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: D345)
Anti-Human Desmoglein-2 (Antigen Aff. Pur.) (Leinco Prod. No.: D350)
Anti-Human Desmoglein-2 (Leinco Prod. No.: D356)