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Recombinant Human Tumor Necrosis Factor Receptor Superfamily Member 14 (TNFRSF14)
Purified No Carrier Protein
(Herpesvirus Entry Mediator (HVEM), TR2, ATAR, HVEA, LIGHTR)
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Prod. No.:H1469
SourceNSO Cells
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Tumor necrosis factor receptor superfamily member 14 (herpesvirus entry mediator), also known as TNFRSF14, is the cellular receptor for TNF superfamily 14 (LIGHT).1 TNFRSF14 is a type I membrane protein that is considered as a Herpesvirus entry mediator.2 It is able to inhibit the proliferation, activation, and cytokine production of T cells. TNFRSF14 is expressed by peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells.

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human TNFRSF14 is Mr 45 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 60 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human HVEM-Fc was determined by its ability to inhibit apoptosis of mouse L-929 cells treated with 0.1 ng/ml of rhTNF-β. The expected ED50 for this effect is typically 0.5 - 2 μg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
palp sckedeypvg seccpkcspg yrvkeacgel tgtvcepcpp gtyiahlngl skclqcqmcd pamglrasrn csrtenavcg cspghfcivq dgdhcaacra yatsspgqrv qkggtesqdt lcqncppgtf spngtleecq hqtkcswlvt kagagtsssh wviegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgkh hhhhh



Country of Origin
USA
References
1. Park JE et al. (2001) Arterioscler Thromb Vasc Biol. 21: 1873
2. Montgomery RI et al. (1996) Cell 87: 427
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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