Anti-Human TNFRSF14

Product No.: H1485


Clone 94801 Target TNFRSF14 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Tumor Necrosis Factor Receptor Superfamily Member 14, Herpesvirus Entry Mediator (HVEM), TR2, ATAR, HVEA, LIGHTR, HVEM Isotype IgG1 Applications CyTOF® , ELISA Cap , FC , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human TNFRSF14 (Accession # AAB58354) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830321 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:H1491 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:H1469. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing HVEM. A suggested method would be to stain cells expressing HVEM with 0.25 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤ 200 µl followed by PN:M1189. Western Blotting: To detect Human TNFRSF14 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TNFRSF14 is 1.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:H1469. Additional Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Tumor Necrosis Factor Receptor Superfamily Member 14 (TNFRSF14) (Clone 94801) recognizes an epitope on Human TNFRSF14. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Tumor necrosis factor receptor superfamily member 14 (herpesvirus entry mediator), also known as TNFRSF14, is the cellular receptor for TNF superfamily 14 (LIGHT).¹ TNFRSF14 is a type I membrane protein that is considered as a Herpesvirus entry mediator.² It is able to inhibit the proliferation, activation, and cytokine production of T cells. TNFRSF14 is expressed by peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. PubMed TNFRSF14 References & Citations 1. Park, JE. et al. (2001) Arterioscler Thromb Vasc Biol. 21: 1873 2. Montgomery, RI. et al. (1996) Cell 87: 427 View product citations for antibody H1485 on CiteAb Technical Protocols CyTOF® Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human TNFRSF14 (Accession # AAB58354)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830321

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:H1491 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:H1469.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing HVEM. A suggested method would be to stain cells expressing HVEM with 0.25 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤ 200 µl followed by PN:M1189.
Western Blotting: To detect Human TNFRSF14 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TNFRSF14 is 1.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:H1469.

Additional Applications Reported In Literature ?

CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Tumor Necrosis Factor Receptor Superfamily Member 14 (TNFRSF14) (Clone 94801) recognizes an epitope on Human TNFRSF14. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Tumor necrosis factor receptor superfamily member 14 (herpesvirus entry mediator), also known as TNFRSF14, is the cellular receptor for TNF superfamily 14 (LIGHT).¹ TNFRSF14 is a type I membrane protein that is considered as a Herpesvirus entry mediator.² It is able to inhibit the proliferation, activation, and cytokine production of T cells. TNFRSF14 is expressed by peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells.

PubMed

TNFRSF14

References & Citations

1. Park, JE. et al. (2001) Arterioscler Thromb Vasc Biol. 21: 1873
2. Montgomery, RI. et al. (1996) Cell 87: 427

View product citations for antibody H1485 on CiteAb

CyTOF®

Certificate of Analysis

Request COA

Prod No.	Description
H1485	Anti-Human TNFRSF14
T310	Anti-Human TNFRSF14 (CT)
H1469	Recombinant Human TNFRSF14
H1491	Anti-Human TNFRSF14 – Biotin

Formats Available

Prod No.	Description
H1485	Anti-Human TNFRSF14

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human TNFRSF14