Product Datasheet
www.leinco.com
excellence in early discovery research       



Recombinant Human Glycoprotein 130 (gp130)
Purified No Carrier Protein
Share |

Size


Prod. No.:G651
SourceNSO Cells
Pkg. Size:100 µg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of tall cytokine receptors. It forms one subunit of type I cytokine receptors within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement.1 Gp130 signals are mediated by tyrosine kinases of the JAK family and by their binding partners, transcription factors of the STAT family.2 Gp130 signaling is very important for osteoblast function and calcium homeostasis.3

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human gp130 is Mr 95 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C for up to three months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human gp130 was determined by its ability to inhibit the IL-6 sR enhancement of IL-6 activity on a mouse myeloid leukemia cell line, M1. The expected ED50 is typically 0.05 - 0.15 μg/ml in the presence of 10 ng/mL rhIL-6 sR and 20 ng/ml rhIL-6.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
The DNA sequence encoding the extracellular domain of Human gp130 (Hibi, M. et al., 1990, Cell 63:1149 - 1157) was fused to the carboxy-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker, inserted into an appropriate mammalian expression vector and was expressed in NS0 cells.
Related Products
Recombinant Human gp130 (Leinco Prod. No.: G645)
PN:
Anti-Human gp130 (Leinco Prod. No.: G666)
Anti-Mouse gp130 (Antigen Aff. Pur.) (Leinco Prod. No.: G668)
Recombinant Mouse gp130 (Leinco Prod. No.: G647)
Anti-Mouse gp130 (Leinco Prod. No.: G671)
Anti-Human gp130 (Leinco Prod. No.: G676)
Anti-Human gp130-Biotin (Antigern Aff. Pur.) (Leinco Prod. No.: G687)
Anti-Mouse gp130-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G690)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
359 Consort Drive | St. Louis, MO 63011 | (800) 538-1145 | (636) 527-5545 fax
800.538.1145 ® Copyright Leinco Technologies, Inc. 2006 – 2012