Recombinant Human Glycoprotein 130 (gp130)

Home/Recombinant Proteins/Recombinant Human Glycoprotein 130 (gp130)

Pricing & Details

G651
Expression Host
NS0 cells
Species
Human
Applications
ELISA Cap
Prod No.
Size
Price
Avail.
Qty
Add to cart
G651-100 µg
100 µg
$410.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Background

Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of tall cytokine receptors. It forms one subunit of type I cytokine receptors within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement.1 Gp130 signals are mediated by tyrosine kinases of the JAK family and by their binding partners, transcription factors of the STAT family.2 Gp130 signaling is very important for osteoblast function and calcium homeostasis.3

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined bythe LAL method
Biological Activity
The biological activity of Human gp130 was determined by its ability to inhibit the IL-6 sR enhancement of IL-6 activity on a mouse myeloid leukemia cell line, M1. The expected ED50 is typically 0.05 - 0.15 μg/ml in the presence of 10 ng/mL rhIL-6 sR and 20 ng/ml rhIL-6.
Fusion Protein Tag
Fc Fusion Protein & 6x His Tag
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
elldpcgyis pespvvqlhs nftavcvlke kcmdyfhvna nyivwktnhf tipkeqytii nrtassvtft diaslniqlt cniltfgqle qnvygitiis glppekpknl scivnegkkm rcewdggret hletnftlks ewathkfadc kakrdtptsc tvdystvyfv nievwveaen algkvtsdhi nfdpvykvkp npphnlsvin seelssilkl twtnpsiksv iilkyniqyr tkdastwsqi ppedtastrs sftvqdlkpf teyvfrircm kedgkgywsd wseeasgity edrpskapsf wykidpshtq gyrtvqlvwk tlppfeangk ildyevtltr wkshlqnytv natkltvnlt ndrylatltv rnlvgksdaa vltipacdfq athpvmdlka fpkdnmlwve wttpresvkk yilewcvlsd kapcitdwqq edgtvhrtyl rgnlaeskcy litvtpvyad gpgspesika ylkqappskg ptvrtkkvgk neavlewdql pvdvqngfir nytifyrtii gnetavnvds shteytlssl tsdtlymvrm aaytdeggkd gpeftfttpk faqgeidieg rmdpkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdeltknqv sltclvkgfy psdiavewes ngqpennykt tppvldsdgs fflyskltvd ksrwqqgnvf scsvmhealh nhytqkslsl spgkhhhhhh
N-terminal Sequence Analysis
Glu23
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human gp130 is Mr 95 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C for up to three months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Bravo, J. et al. (1998) EMBO J 17: 1665
2. Graeve, L et al. (1998) Biochem J 334: 297
3. Kronenber, HM. et al. (2004) Endocrinology 145: 1376
Elisa Sandwich Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Leinco Technologies uses cookies to improve your experience and our website service. By continuing to browse our website, you accept our cookie policy. Ok