Recombinant Human Glycoprotein 130 (gp130)

Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of tall cytokine receptors. It forms one subunit of type I cytokine receptors within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement.¹ Gp130 signals are mediated by tyrosine kinases of the JAK family and by their binding partners, transcription factors of the STAT family.² Gp130 signaling is very important for osteoblast function and calcium homeostasis.³

The biological activity of Human gp130 was determined by its ability to inhibit the IL-6 sR enhancement of IL-6 activity on a mouse myeloid leukemia cell line, M1. The expected ED₅₀ is typically 0.05 - 0.15 μg/ml in the presence of 10 ng/mL rhIL-6 sR and 20 ng/ml rhIL-6.

Fc Fusion Protein & 6x His Tag

elldpcgyis pespvvqlhs nftavcvlke kcmdyfhvna nyivwktnhf tipkeqytii nrtassvtft diaslniqlt cniltfgqle qnvygitiis glppekpknl scivnegkkm rcewdggret hletnftlks ewathkfadc kakrdtptsc tvdystvyfv nievwveaen algkvtsdhi nfdpvykvkp npphnlsvin seelssilkl twtnpsiksv iilkyniqyr tkdastwsqi ppedtastrs sftvqdlkpf teyvfrircm kedgkgywsd wseeasgity edrpskapsf wykidpshtq gyrtvqlvwk tlppfeangk ildyevtltr wkshlqnytv natkltvnlt ndrylatltv rnlvgksdaa vltipacdfq athpvmdlka fpkdnmlwve wttpresvkk yilewcvlsd kapcitdwqq edgtvhrtyl rgnlaeskcy litvtpvyad gpgspesika ylkqappskg ptvrtkkvgk neavlewdql pvdvqngfir nytifyrtii gnetavnvds shteytlssl tsdtlymvrm aaytdeggkd gpeftfttpk faqgeidieg rmdpkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdeltknqv sltclvkgfy psdiavewes ngqpennykt tppvldsdgs fflyskltvd ksrwqqgnvf scsvmhealh nhytqkslsl spgkhhhhhh

Glu23

Lyophilized

The predicted molecular weight of Recombinant Human gp130 is Mr 95 kDa.

95

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C for up to three months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

1. Bravo, J. et al. (1998) EMBO J 17: 1665
2. Graeve, L et al. (1998) Biochem J 334: 297
3. Kronenber, HM. et al. (2004) Endocrinology 145: 1376