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Rabbit Anti-Human B-Cell Activating Factor (BAFF-R)
Purified No Carrier Protein
(TNFRSF13C, BAFF-R, BAFFR, CD268, MGC138235)
Polyclonal Antibody
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Prod. No.:B514
Host:Rabbit
Conc.:1.0 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human B Cell Activating Factor (BAFF-R) recognizes Human BAFF-R. This polyclonal antibody was purified using affinity chromatography.

Background:
Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF, BLyS, TALL-1, THANK, and zTNF4.1-4 BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion.1,2 Two receptors, TACI and BCMA, for BAFF were originally identified. A third receptor was identified recently and designated BAFF-R and BR3 for BLyS receptor 3. Unlike BCMA and TACI, which bind to BAFF and April, BAFF-R/BR3 is specific for BAFF and plays a predominant role in BAFF induced B cell development and survival. BAFF and its receptors are involved in B cell associated autoimmune diseases, and activate NF-kB and c-jun N-terminal kinase.4

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Purified Recombinant Human BAFF-R (>98%)
Blocking Peptide
BAFF-R (CT) Blocking Peptide (Leinco Prod. No.: B504)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB, ICC
Western Blotting: To detect Human BAFF-R this polyclonal antibody can be used at a concentration of 2-5 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Human Spleen Tissue Lysate (Leinco Prod. No.: H1410).
Immunocytochemistry: 5 µg/ml
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Moore, P. A. et al. (1999) Science 285:260
2. Schneider, P. et al. (1999) J. Exp. Med. 189:1747
3. Shu, H. B. et al. (1999) J. Leukoc. Biol. 65:680
4. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978
Related Products
BAFF-R (CT) Blocking Peptide (Leinco Prod. No.: B504)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Human Spleen Tissue Lysate (Leinco Prod. No.: H1410)
Anti-Human BAFF (Leinco Prod. No.: B570)
Anti-Human BAFF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B417)
Recombinant Human BAFF-R (Leinco Prod. No.: B131)
Recombinant Human BAFF (Leinco Prod. No.: B115)
BAFF (CT) Blocking Peptide (Leinco Prod. No.: B368)
Anti-Human BAFF (CT) (Leinco Prod. No.: B359)
Anti-Human BAFF (Leinco Prod. No.: B379)
Anti-Human BAFF (Antigen Aff. Pur.) (Leinco Prod. No.: B416)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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