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Goat Anti-Human Glycoprotein 130 (gp130)
Biotin
(IL6ST, IL6β, CD130, CDw130, GP130, GP130-RAPS, IL6R-Beta)
Polyclonal Antibody
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Prod. No.:G687
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human Glycoprotein 130 (gp130) recognizes Human gp130. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of tall cytokine receptors. It forms one subunit of type I cytokine receptors within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement.1 Gp130 signals are mediated by tyrosine kinases of the JAK family and by their binding partners, transcription factors of the STAT family.2

Gp130 signaling is very important for osteoblast function and calcium homeostasis.3

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human gp130 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB,
Western Blotting: To detect Human gp130 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human gp130 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human gp130 (Leinco Prod. No.: G651).
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with Anti-Human gp130 (Leinco Prod. No.: G676) as the capture antibody at 0.4 µg/ml and an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) for the detection of Human gp130.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Bravo et al. (1998) EMBO J 17: 1665
2. Graeve, L et al. (1998) Biochem J 334: 297
3. Henry M. Kronenber et al. (2004) Endocrinology 145: 1376
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human gp130 (Leinco Prod. No.: G651)
Anti-Human gp130 (Leinco Prod. No.: G676)
Recombinant Human gp130 (Leinco Prod. No.: G645)
PN:
Anti-Human gp130 (Leinco Prod. No.: G666)
Anti-Mouse gp130 (Antigen Aff. Pur.) (Leinco Prod. No.: G668)
Recombinant Mouse gp130 (Leinco Prod. No.: G647)
Anti-Mouse gp130 (Leinco Prod. No.: G671)
Anti-Mouse gp130-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G690)
UltraAvadin™ Reagents
Streptavadin Reagents
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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