Anti-Mouse gp130 – Biotin
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen NS0-Derived Recombinant Mouse gp130 Extracellular Domain (Accession # Q6PDI9) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830279 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing gp130. A suggested method would be to stain cells expressing gp130 with 3 - 10 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:A104.Western Blotting: To detect Mouse gp130 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse gp130 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL. Use in combination with: Capture Reagent: Mouse gp130 Antibody. Standard: Recombinant Mouse gp130 Fc Chimera Protein, CF. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Glycoprotein 130 (gp130) recognizes Mouse gp130. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of tall cytokine receptors. It forms one subunit of type I cytokine receptors within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement.1 Gp130 signals are mediated by tyrosine kinases of the JAK family and by their binding partners, transcription factors of the STAT family.2 Gp130 signaling is very important for osteoblast function and calcium homeostasis.3 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Bravo, J. et al. (1998) EMBO J 17: 1665 2. Graeve, L et al. (1998) Biochem J 334: 297 3. Kronenber, HM. et al. (2004) Endocrinology 145: 1376 Technical ProtocolsELISA Det   |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
