Anti-Human gp130 (Clone 28105)

Anti-Human gp130 (Clone 28105)

Product No.: G676

[product_table name="All Top" skus="G676"]

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Clone
28105
Target
gp130
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Glycoprotein 130, IL6ST, IL6β, CD130
Isotype
IgG1
Applications
ELISA Cap
,
IHC
,
N

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human gp130 (Accession # P40189)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/mL. A suitable detection antibody is PN:G687 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:G651.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human gp130 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human gp130 (at 0.8 ng/ml) is 0.02 - 0.04 µg/ml.
Immunohistochemistry: Suitable for use at concentration of 5-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human gp130 (Clone 28105) recognizes an epitope on Human gp130. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Glycoprotein 130 is a transmembrane protein which is the founding member of the class of tall cytokine receptors. It forms one subunit of type I cytokine receptors within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement. As with other type I cytokine receptors, gp130 possesses a WSXWS amino acid motif that ensures correct protein folding and ligand binding. It interacts with Janus kinases to elicit an intracellular signal following receptor interaction with its ligand. Structurally, gp130 is composed of five fibronectin type-III domains and one immunoglobulin-like C2-type (immunoglobulin-like) domain in its extracellular portion. The members of the IL-6 receptor family all complex with gp130 for signal transduction. For example, IL-6 binds to the IL-6 Receptor. The complex of these two proteins then associates with gp130. This complex of 3 proteins then homodimerizes to form a hexameric complex which can produce downstream signals. There are many other proteins which associate with gp130, such as cardiotrophin 1 (CT-1), leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), and IL-11. There are also several other proteins which have structural similarity to gp130 and contain the WSXWS motif and preserved cysteine residues. Members of this group include LIF-R, OSM-R, and G-CSF-R.

Antigen Details

PubMed

References & Citations

1. Bravo, J. et al. (1998) EMBO J 17: 1665
2. Graeve, L et al. (1998) Biochem J 334: 297
3. Kronenber, HM. et al. (2004) Endocrinology 145: 1376
Elisa Sandwich Protocol
IHC
N

Certificate of Analysis

Formats Available

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Prod No.
Description
G676
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.