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Streptavidin-Horseradish Peroxidase (HRPO)
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Prod. No.:S207
Conc.:1.0 mg/ml
Pkg. Size:1.0 mg, 2.0 mg
Description
Background:
Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10 -15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.

Reporter Molecule:
Horseradish Peroxidase (HRPO) is an enzyme purified from horseradish root with a molecular weight of approximately 44 kDa.

Formulation
Streptavidin-HRPO is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA, and 0.01% thimerosol as a preservative. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)
Storage and Stability
Stable when stored at 2 - 8°C. Do not freeze
Applications and Suggested Working Dilutions
Western blotting: 1:5,000 - 1:10,000
ELISA: 1:5,000 - 1:20,000
Immunohistochemistry: 1:100 - 1:1,000
Western blotting and ELISA Chemiluminescent: 1:20,000 - 1:100,000

However, each investigator should determine their own optimal working dilution for each specific research application.
Country of Origin
USA
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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