UltraAvidin™- Horseradish Peroxidase (HRP)

Pricing & Details

Product No.A106
Formats AvailableView All
Product Type
Avidin - Biotin
Prod No.
Size
Price
Avail.
Qty
Add to cart
A106-2.0 mg
2.0 mg
$125.00
In stock
Max:
Min: 1
Step: 1
A106-5.0 mg
5.0 mg
$275.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Host Species
Chicken Egg White
Product Concentration
1.0 mg/ml
Formulation
UltraAvidin-HRP is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA, and 0.01% thimerosol as a preservative. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)
Storage and Handling
Stable when stored at 2 - 8°C. Do not freeze
Country of Origin
USA
Working Concentration
Western blotting: 1:5,000 - 1:10,000
ELISA: 1:5,000 - 1:20,000
Immunohistochemistry: 1:100 - 1:1,000
Western blotting and ELISA Chemiluminescent: 1:20,000 - 1:100,000

However, each investigator should determine their own optimal working dilution for each specific research application.
Isoelectric Point
pI= 6.3 +/- 0.3
Specific Activity
11-17 µg biotin bound/mg UltraAvidin™
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Background
UltraAvidin is Leinco Technologies' trademark for a uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000 there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin has a near neutral pI which prevents electrostatic interactions with negatively charged serum or membrane proteins.

Recent studies have identified a universal recognition sequence in streptavidin which is similar to that found in several adhesion receptors.1 Strong interactions have been shown with streptavidin and cell surface molecules. This universal recognition sequence is not present in UltraAvidin.

UltraAvidin has been covalently conjugated to Horseradish Peroxidase. The conjugate is then purified chromatographically to remove unconjugated enzyme and UltraAvidin.

References & Citations

1. Wilchek, M. et al.(1990) Biochem Biophys Res Commun. 1990 170(3):1236-41.
2. Sullivan, W. et al. (1996) Genetics. 143(4):1629-42.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.