450 nm Stop Reagent for TMB Microwell

450 nm Stop Reagent for TMB Microwell

Product No.: T125

[product_table name="All Top" skus="T125"]

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Product Type
Substrate and Assay Reagents, Reagents for Assay Development
Applications
ELISA
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Microwell

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Product Details

Storage and Handling
Store at room temperature (25ºC). Refrigerated temperatures will not harm the reagent.
Country of Origin
USA
Shipping
Next Day Ambient
Expiration Date
Stable for a minimum of 3 years from the date of manufacture when properly stored at room temperature.

Description

Background
450 nm Liquid Stop Solution for TMB Microwell Substrates is a proprietary acidic formulation used to stop TMB Microwell Substrate (Leinco Prod. No.: T118) reactions and change the chromogen from blue to yellow in color.
Directions for Use
450 nm Stop Reagent for TMB Microwell Substrates is used to stop Leinco Technologies’ TMB Microwell Substrates and change the absorbance from blue (620 nm) to yellow (450 nm). 450 nm Stop Reagent for TMB Microwell Substrates requires reconstitution with 100 ml of distilled or deionized water. Allow approximately 5 minutes for the solid to dissolve. Equal volumes of Leinco’s TMB Substrate and 450 nm Stop Reagent for TMB Microwell Substrates should be used. Stopping the TMB substrate reaction increases the sensitivity 2 – 4 fold.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Hazard Description
CAUTION! This is an acidic formulation and should be treated with the same precautions as any other acid. Refer to the SDS for additional information.

Related Protocols

Elisa Sandwich Protocol
Microwell
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.