Specificity:

Mouse Anti-Human CD2 (Clone G11) recognizes Human CD2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD2 recognizes a cell surface glycoprotein (Mr 45-59 kDa) on human lymphocytes. This antigen also forms the binding site for sheep erythrocytes.

The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.

Background:

Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function.¹ The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58),² the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR).³ Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.