Mouse Anti-Human CD2 (Clone G11) recognizes an epitope on Human CD2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.
Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function.1 The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58),2 the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR).3 Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.
DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Known Species Cross Reactivity: Hu+
Purified Recombinant Human CD2 (>98%)
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
1. Meuer, S.C. et al. (1984) Cell36:897
2. Peterson, A. and Sneed, B. (1987) Nature329:842
3. Bierer, B.E. et al.(1989) Annu. Rev Immunol.7:579
4. Krensky, C.F. et al. (1982) Proc. Natl. Acad. Sci. USA79:7489
5. Krensky, C.F. et al. (1983) J. Immunol.131:611