Product Datasheet
www.leinco.com
excellence in early discovery research       



Recombinant Human Chemokine (C-C Motif) Ligand 25 (CCL25)
Purified No Carrier Protein
(SCYA25, TECK, Ckb15)
Share |


Prod. No.:T165
SourceE. coli
Pkg. Size:25 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.1 It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.2 CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.3

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human CCL25 is Mr 14.3 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Thymus-Expressed Chemokine is determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mqgvfedc clayhypigw avlrrawtyr iqevsgscnl paaifylpkr hrkvcgnpks revqramkll darnkvfakl hhnmqtfqag phavkklssg nsklssskfs npissskrnv sllisansgl



Country of Origin
USA
References
1. Vacari et al. (1997) Immunity 7: 291
2. Agace W et al. (2006) J Immunol. 176: 3642
3. Teixidó J. et al. (2007) J Leukoc Biol. 82: 380
Related Products
Anti-Human CCL25 (Leinco Prod. No.: T216)
Anti-Human CCL25-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T185)
Anti-Human CCL25 (Antigen Aff. Pur.) (Leinco Prod. No.: T167)
Recombinant Mouse TECK (Leinco Prod. No.: T396)
Anti-Human CCL25 (Leinco Prod. No.: C1399)
Anti-Mouse CCL25 (Leinco Prod. No.: C1428)
PN:
Anti-Mouse CCL25-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T491)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
359 Consort Drive | St. Louis, MO 63011 | (800) 538-1145 | (636) 527-5545 fax
800.538.1145 ® Copyright Leinco Technologies, Inc. 2006 – 2012