Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.1 It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.2 CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.3
>95% by SDS-PAGE and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
The biological activity of Human Thymus-Expressed Chemokine is determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.
Protein Accession No.
Amino Acid Sequence
qgvfedc clayhypigw avlrrawtyr iqevsgscnl paaifylpkr hrkvcgnpks revqramkll darnkvfakl hhnmqtfqag phavkklssg nsklssskfs npissskrnv sllisansgl
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CCL25 is Mr 14 kDa. Additionally, the actual molecular weight as observed by migration on SDS-PAGE is Mr 14 kDa.
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Zlotnik, A. et al. (1997) Immunity. 7(2):291-301.
2. Agace, W. et al. (2006) J Immunol. 176:3642
3. Teixidó, J. et al. (2007) J Leukoc Biol. 82:380
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.