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Recombinant Mouse Glycoprotein 130 (gp130)
Purified No Carrier Protein
(IL6ST, IL6β, CD130, CDw130, GP130, GP130-RAPS, IL6R-Beta)
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Prod. No.:G647
SourceNSO Cells
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of tall cytokine receptors. It forms one subunit of type I cytokine receptors within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement.1 Gp130 signals are mediated by tyrosine kinases of the JAK family and by their binding partners, transcription factors of the STAT family.2 Gp130 signaling is very important for osteoblast function and calcium homeostasis.3

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse gp130 is Mr 94 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 130 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse gp130 was determined by by its ability to inhibit the IL-6 sR enhancement of IL-6 activity on a mouse myeloid leukemia cell line, M1 (Saito, T. et al., 1991, J. Immunol. 147:168). The expected ED50 for this effect is typically 0.03-0.10 mg/ml in the presence of 10 ng/ml rhIL-6 sR and 20 ng/ml rhIL-6.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
qllepcgy iypefpvvqr gsnftaicvl keaclqhyyv nasyivwktn haavpreqvt vinrttssvt ftdvvlpsvq ltcnilsfgq ieqnvygvtm lsgfppdkpt nltcivnegk nmlcqwdpgr etyletnytl ksewatekfp dcqskhgtsc mvsymptyyv nievwveaen algkvssesi nfdpvdkvkp tppynlsvtn seelssilkl swvssglggl ldlksdiqyr tkdastwiqv pledtmsprt sftvqdlkpf teyvfrirsi kdsgkgywsd wseeasgtty edrpsrppsf wyktnpshgq eyrsvrliwk alplseangk ildyeviltq sksvsqtytv tgteltvnlt ndryvaslaa rnkvgksaaa vltipsphvt aaysvvnlka fpkdnllwve wtpppkpvsk yilewcvlse napcvedwqq edatvnrthl rgrlleskcy qitvtpvfat gpggseslka ylkqaaparg ptvrtkkvgk neavlawdqi pvddqngfir nysisyrtsv gkemvvhvds shteytlssl ssdtlymvrm aaytdeggkd gpeftfttpk faqgeiedie grmdpkscdk thtcppcpap ellggpsvfl fppkpkdtlm isrtpevtcv vvdvshedpe vkfnwyvdgv evhnaktkpr eeqynstyrv vsvltvlhqd wlngkeykck vsnkalpapi ektiskakgq prepqvytlp psrdeltknq vsltclvkgf ypsdiavewe sngqpennyk ttppvldsdg sfflyskltv dksrwqqgnv fscsvmheal hnhytqksls lspgkhhhhh h



Country of Origin
USA
References
1. Bravo et al. (1998) EMBO J 17: 1665
2. Graeve, L et al. (1998) Biochem J 334: 297
3. Henry M. Kronenber et al. (2004) Endocrinology 145: 1376
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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