IHC (NBF/Par.): This Antibody has not been tested by Leinco for the use in immunohistochemistry. However, the antibody was used in reference 1 for immunohistochemistry.

Western Blotting: To detect Human KRT19 this monoclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Immunocytochemistry: This antibody was used at a concentration of 10 μg/mL to detect Cytokeratin 19 on human MDA-MB 231 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 45 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 1 hour at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine Red-coupled anti-mouse IgG at room temperature covered with foil for one hour. Between each step, cells were washed with Leinco Prod. No. W101.