Anti-Human Cytokeratin 19 – Purified

Pricing & Details

Product No.C162
Clone
BA17
Protein
Cytokeratin 19
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
PTPN22, PTPN8 (former), PEP
Isotype
Mouse
IgG1
Applications
ICC
,
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C162-0.2 mg
0.2 mg
$198.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Mammary organoids
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage
IHC (NBF/Par.): This Antibody has not been tested by Leinco for the use in immunohistochemistry. However, the antibody was used in reference 1 for immunohistochemistry.
Western Blotting: To detect Human KRT19 this monoclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Immunocytochemistry: This antibody was used at a concentration of 10 μg/mL to detect Cytokeratin 19 on human MDA-MB 231 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 45 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 1 hour at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine Red-coupled anti-mouse IgG at room temperature covered with foil for one hour. Between each step, cells were washed with Leinco Prod. No. W101.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Human Cytokeratin 19 (clone BA17) reacts with the smallest human cytokeratin (Mr 40 kDa).1 The antibody reacts specifically with cytokeratin 19 and has no reactivity with other cytokeratins.
Antigen Distribution
Anti-Cytokeratin 19 reacts strongly with luminal (secretory) epithelial cells but does not stain the basal (myoepithelial) cells. There is a subset of secretory epithelial cells which were Keratin 19- and are thought to be precursors to keratin 19+ cells.1,2
Background
Cytokeratin 19, a type I intermediate filament protein, it is the smallest known acidic cytokeratin. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours.

Antigen Details

Research Area
Cell Biology
.
Neuroscience
.
Neuroscience Cell Markers

References & Citations

1. Bartek J. et al. (1985) J. Cell Sci. 75:17 2. Bartek J. et al. (1985) Int. J. Cancer 36:299
IHC
General Western Blot Protocol

Formats Available

Prod No.
Description
C162
Products are for research use only. Not for use in diagnostic or therapeutic procedures.