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Goat Anti-Human PDGF-BB (PDGF-BB)
Biotin
(Glioma Derived Growth Factor [GDGF], Osteosarcoma Derived Growth Factor [ODGF])
Polyclonal Antibody
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Prod. No.:P130
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human Platelet Derived Growth Factor-BB (PDGF-BB) recognizes Human PDGF-BB. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Platelet-derived growth factor-BB homodimers (PDGF-BB) activates all combinations of PDGF receptor subunits, whereas PDGF-AA acts specifically on PDGFR alpha.1 This constitutes a positive feed-back mechanism, which, for example, could serve to potentiate autocrine stimulation of growth.2 PDGF-BB plays an important role in the cellular metabolism of vascular wall by regulating the rate of macrophage-colony stimulating factor (M-CSF) production in vascular smooth muscle cells.3 PDGF-BB is also a potent wound-healing hormones that accelerate incisional repair.4 PDGF-BB is associated with excessive cell migration, proliferation and many growth-related diseases.5 It may be a new prognostic indicator for patients with colorectal cancer.6 PDGF-BB may also induce coronary atherosclerotic disease formation by stimulating coronary artery smooth muscle cell (CASMC) migration and proliferation through agent-specific regulation of oxidative status and utilization of different signal transduction pathways.5

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human PDGF-BB (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB,
Western Blotting: To detect Human PDGF-BB this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human PDGF-BB is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human PDGF-BB (Leinco Prod. No.: P126).
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Josef Pfeilschifter et al. (2005) Am Society of Nephrol. 10: 1681
2. Claesson-Welsh L. et al. (1991) J Biol Chem. 266: 21138
3. Yamada N et al. (1992) J Biol Chem. 267: 15455
4. Mustoe TA et al. (1991) J Lab Clin Med. 117: 373
5. Bayraktutan U. et al. (2008) J Mol Cell Cardiol. 45: 198
6. Mori M et al. (2008) Ann Surg Oncol. 15: 2129
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human PDGF-BB (Leinco Prod. No.: P126)
Recombinant Mouse PDGF-BB (Leinco Prod. No.: P243)
Recombinant Rat PDGF-BB (Leinco Prod. No.: P256)
Human PDGF-BB ELISA Development Kit (Leinco Prod. No.: P134)
Anti-Human PDGF-BB (Antigen Aff. Pur.) (Leinco Prod. No.: P122)
UltraAvadin™ Reagents
Streptavadin Reagents
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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