Recombinant Rat PDGF-BB

Platelet-derived growth factor-BB homodimers (PDGF-BB) activates all combinations of PDGF receptor subunits, whereas PDGF-AA acts specifically on PDGFR alpha.¹ This constitutes a positive feed-back mechanism, which, for example, could serve to potentiate autocrine stimulation of growth.² PDGF-BB plays an important role in the cellular metabolism of vascular wall by regulating the rate of macrophage-colony stimulating factor (M-CSF) production in vascular smooth muscle cells.³ PDGF-BB is also a potent wound-healing hormones that accelerate incisional repair.⁴ PDGF-BB is associated with excessive cell migration, proliferation and many growth-related diseases.⁵ It may be a new prognostic indicator for patients with colorectal cancer.⁶ PDGF-BB may also induce coronary atherosclerotic disease formation by stimulating coronary artery smooth muscle cell (CASMC) migration and proliferation through agent-specific regulation of oxidative status and utilization of different signal transduction pathways.⁵

The biological activity of Rat PDGF-BB was determined by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts (Raines, E. W. et al., 1985, Methods in Enzymology 109:749-773). The expected ED₅₀ for this effect is typically 1 - 3 ng/ml.

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Ser74

Lyophilized

The predicted molecular weight of Recombinant Rat PDGF-BB is Mr 25 kDa.

25

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Pfeilschifter, J. et al. (2005) Am Society of Nephrol. 10: 1681
2. Claesson-Welsh, L. et al. (1991) J Biol Chem. 266: 21138
3. Yamada, N. et al. (1992) J Biol Chem. 267: 15455
4. Mustoe, TA. et al. (1991) J Lab Clin Med. 117: 373
5. Bayraktutan, U. et al. (2008) J Mol Cell Cardiol. 45: 198
6. Mori, M. et al. (2008) Ann Surg Oncol. 15: 2129