Anti-Human PDGF-BB

Anti-Human PDGF-BB

Product No.: P122

[product_table name="All Top" skus="P122"]

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Target
PDGF-BB
Product Type
Polyclonal Antibody
Alternate Names
Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)
Applications
N
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WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human PDGF-BB (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Additional Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize PDGF‑BB-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Raines, E.W. et al. (1985) Methods Enzymol. 109:749]. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑BB.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Platelet Derived Growth Factor-BB (PDGF-BB) recognizes Human PDGF-BB. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Platelet-derived growth factor-BB homodimers (PDGF-BB) activates all combinations of PDGF receptor subunits, whereas PDGF-AA acts specifically on PDGFR alpha.1 This constitutes a positive feed-back mechanism, which, for example, could serve to potentiate autocrine stimulation of growth.2 PDGF-BB plays an important role in the cellular metabolism of vascular wall by regulating the rate of macrophage-colony stimulating factor (M-CSF) production in vascular smooth muscle cells.3 PDGF-BB is also a potent wound-healing hormones that accelerate incisional repair.4 PDGF-BB is associated with excessive cell migration, proliferation and many growth-related diseases.5 It may be a new prognostic indicator for patients with colorectal cancer.6 PDGF-BB may also induce coronary atherosclerotic disease formation by stimulating coronary artery smooth muscle cell (CASMC) migration and proliferation through agent-specific regulation of oxidative status and utilization of different signal transduction pathways.5

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Pfeilschifter, J. et al. (2005) Am Society of Nephrol. 10: 1681
2. Claesson-Welsh, L. et al. (1991) J Biol Chem. 266: 21138
3. Yamada, N. et al. (1992) J Biol Chem. 267: 15455
4. Mustoe, TA. et al. (1991) J Lab Clin Med. 117: 373
5. Bayraktutan, U. et al. (2008) J Mol Cell Cardiol. 45: 198 6. Mori, M. et al. (2008) Ann Surg Oncol. 15: 2129
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General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.