Recombinant Mouse PDGF-BB

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Pricing & Details

Product. No.P243
Alternate Names
Glioma-derived growth factor [GDGF], Osteosarcoma-derived Growth Factor [ODGF]
Expression Host
E. coli
Species
Mouse
Prod No.
Size
Price
Avail.
Qty
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P243-10 µg
10 µg
$224.00
In stock
Max:
Min: 1
Step: 1
P243-100 µg
100 µg
$581.00
In stock
Max:
Min: 1
Step: 1
P243-500 µg
500 µg
$2,524.00
In stock
Max:
Min: 1
Step: 1
P243-1 mg
1 mg
$4,163.00
In stock
Max:
Min: 1
Step: 1

Background

Platelet-derived growth factor-BB homodimers (PDGF-BB) activates all combinations of PDGF receptor subunits, whereas PDGF-AA acts specifically on PDGFR alpha.1 This constitutes a positive feed-back mechanism, which, for example, could serve to potentiate autocrine stimulation of growth.2 PDGF-BB plays an important role in the cellular metabolism of vascular wall by regulating the rate of macrophage-colony stimulating factor (M-CSF) production in vascular smooth muscle cells.3 PDGF-BB is also a potent wound-healing hormones that accelerate incisional repair.4 PDGF-BB is associated with excessive cell migration, proliferation and many growth-related diseases.5 It may be a new prognostic indicator for patients with colorectal cancer.6 PDGF-BB may also induce coronary atherosclerotic disease formation by stimulating coronary artery smooth muscle cell (CASMC) migration and proliferation through agent-specific regulation of oxidative status and utilization of different signal transduction pathways.5

Protein Details

Purity
>98% by SDS Page and HPLC
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of the disulfide linked homodimer Recombinant Mouse PDGF-BB is Mr 24.6 kDa.
Formulation
This protein was 0.2 µm filtered and lyophilized from Sodium Citrate, pH 3.0.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Pfeilschifter, J. et al. (2005) Am Society of Nephrol. 10: 1681
2. Claesson-Welsh, L. et al. (1991) J Biol Chem. 266: 21138
3. Yamada, N. et al. (1992) J Biol Chem. 267: 15455
4. Mustoe, TA. et al. (1991) J Lab Clin Med. 117: 373
5. Bayraktutan, U. et al. (2008) J Mol Cell Cardiol. 45: 198
6. Mori, M. et al. (2008) Ann Surg Oncol. 15: 2129
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.