Product Datasheet
www.leinco.com
excellence in early discovery research       



Anti-Mouse Chemokine (C-C Motif) Ligand 25 (CCL25)
Purified No Carrier Protein
(SCYA25, TECK, Ckb15)
Monoclonal Antibody
Share |


Prod. No.:C1428
Clone:89818
Isotype:Rat IgG2b
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CCL25 (Clone 89818) recognizes an epitope on Mouse CCL25. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.1 It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.2 CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.3

Host Species
Rat
Immunogen
E. coli-Derived Recombinant Mouse CCL25
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (Leinco Prod. No.: R380). The detection sensitivity of this indirect ELISA for Mouse CCL25 is approximately 10 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Rat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1215). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Mouse CCL25 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CCL25 (at 1.6 ng/ml) is 1.5 - 6 µg/ml.
Western Blotting: To detect Mouse CCL25 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse CCL25 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Vacari et al. (1997) Immunity 7: 291
2. Agace W et al. (2006) J Immunol. 176: 3642
3. Teixidó J. et al. (2007) J Leukoc Biol. 82: 380
Related Products
Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (Leinco Prod. No.: R380)
Goat Anti-Rat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1215)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CCL25 (Leinco Prod. No.: T216)
Anti-Human CCL25-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T185)
Anti-Human CCL25 (Antigen Aff. Pur.) (Leinco Prod. No.: T167)
Recombinant Mouse TECK (Leinco Prod. No.: T396)
Anti-Human CCL25 (Leinco Prod. No.: C1399)
PN:
Recombinant Human CCL25 (Leinco Prod. No.: T165)
Anti-Mouse CCL25-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T491)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
359 Consort Drive | St. Louis, MO 63011 | (800) 538-1145 | (636) 527-5545 fax
800.538.1145 ® Copyright Leinco Technologies, Inc. 2006 – 2012