Anti-Human CCL25 (Clone 52529)

Anti-Human CCL25 (Clone 52529)

Product No.: C1399

[product_table name="All Top" skus="C1399"]

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Clone
52529
Target
CCL25
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Chemokine (C-C Motif) Ligand 25, SCYA25, TECK, Ckb15
Isotype
IgG1
Applications
ELISA Cap
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CCL25 (Accession # AAB69981)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T185 at a concentration of approximately 200 ng/ml. A suggested standard for this assay is PN:T165.
Western Blotting: To detect Human CCL25 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL25 is 0.1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CCL25 (Clone 52529) recognizes an epitope on Human CCL25. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.1 It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.2 CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.3

Antigen Details

PubMed

References & Citations

1. Zlotnik, A. et al. (1997) Immunity. 7(2):291-301.
2. Agace, W. et al. (2006) J Immunol. 176:3642
3. Teixidó, J. et al. (2007) J Leukoc Biol. 82:380
Elisa Sandwich Protocol
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
C1399
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.