Anti-Mouse CCL25 (Clone 89818)

Pricing & Details

Product No.C1428
Clone
89818
Protein
CCL25
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
SCYA25, TECK, Ckb15, Chemokine (C-C Motif) Ligand 25
Isotype
Rat
IgG2b
Applications
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1428-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
C1428-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
C1428-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
E. coli-Derived Recombinant Mouse CCL25
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse CCL25 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CCL25 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse CCL25 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CCL25 (at 1.6 ng/ml) is 1.5 - 6 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse CCL25 (Clone 89818) recognizes an epitope on Mouse CCL25. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.1 It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.2 CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.3

Antigen Details

Protein
PubMed

References & Citations

1. Zlotnik, A. et al. (1997) Immunity. 7(2):291-301.
2. Agace, W. et al. (2006) J Immunol. 176:3642
3. Teixidó, J. et al. (2007) J Leukoc Biol. 82:380
General Western Blot Protocol

Formats Available

Prod No.
Description
C1428
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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