Anti-Human CCL25-Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen E. coli-Derived Recombinant Human TECK Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831837 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human CCL25 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL25 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T165. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 200 ng/ml when used in conjunction with PN:C1399 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human CCL25. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Chemokine (C-C Motif) Ligand 25 (CCL25) recognizes Human CCL25. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.1 It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.2 CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.3 PubMed NCBI Gene Bank ID UniProt.org Research Area Chemokine References & Citations1. Zlotnik, A. et al. (1997) Immunity. 7(2):291-301. 2. Agace, W. et al. (2006) J Immunol. 176:3642 3. Teixidó, J. et al. (2007) J Leukoc Biol. 82:380 Technical ProtocolsELISA Det  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
