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Anti-Mouse CD90 (CD90)
Purified
(Thy-1)
Monoclonal Antibody
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Prod. No.:C279
Clone:HK2.1
Isotype:Rat IgG2c
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 500 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CD90 (Clone HK2.1) recognizes the Thy-1.1 and Thy-1.2 alloantigens of all mouse strains tested. This antibody is reported to stimulate mouse T-cell proliferation.1

Antigen Distribution:
Anti-Mouse CD90 (Thy-1) is present on thymocytes, most peripheral T-lymphocytes, some intraepithelial T-lymphocytes, epithelial cells, fibroblasts, neurons and hematopoietic stem cells, but not B-cells of most mice tested.1,2

Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable at 2-8°C. Do not freeze.
Reported Applications
IHC (Frozen), FC, FA
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD90. A suggested method would be to stain cells expressing CD90 with 1 µg per 1 x 106 cells in a total staining volume of 100 µl followed by Goat F(ab')2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin (Leinco Prod. No.: R485).
Functional Assay: It is recommended to use Anti-Mouse CD90 (Thy 1) (Activating) Low Endotoxin Functional Formulation (Leinco Prod. No.: C335) for functional studies.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Ledbetter, J. A. et al. (1979) Immunol. Rev. 47:63
2. Ledbetter, J. A. et al. (1980) J. Exp. Med. 152:280
3. Lancki, D. W. et al. (1984) Immunol. Rev. 81:65
Related Products
Goat F(ab')2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin (Leinco Prod. No.: R485)
Anti-Mouse CD90 (Thy 1) (Activating) Low Endotoxin Functional Formulation (Leinco Prod. No.: C335)
Anti-Human CD90 (Thy-1) Purified (Leinco Prod. No.: C749)
Anti-Mouse CD90 (Thy 1, non-polymorphic) Purified (Leinco Prod. No.: C271)
Anti-Mouse CD90 (Thy 1, non-polymorphic)-Biotin (Leinco Prod. No.: C272)
Anti-Mouse CD90 (Thy 1, non-polymorphic)-Fluorescein (Leinco Prod. No.: C273)
Anti-Mouse CD90 (Thy 1) (Activating)-Fluorescein (Leinco Prod. No.: C281)
Anti-Mouse CD90 (Thy 1.2) Purified (Leinco Prod. No.: C283)
Anti-Mouse CD90 (Thy 1.2)-Biotin (Leinco Prod. No.: C284)
Anti-Mouse CD90 (Thy 1.2)-R-phycoerythrin (Leinco Prod. No.: C286)
Anti-Mouse CD90 (Thy 1) (Activating)-Allophycocyanin (Leinco Prod. No.: C302)
Anti-Mouse CD90 (Thy 1, non-polymorphic)-Allophycocyanin (Leinco Prod. No.: C356)
Anti-Mouse CD90 (Thy 1, non-polymorphic)-R-phycoerythrin (Leinco Prod. No.: C274)
Anti-Mouse CD90 (Thy 1, non-polymorphic) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C392)
Anti-Mouse CD90 (Thy 1.2) Low Endotoxin Functional Formulation (Leinco Prod. No.: C393)
Anti-Human CD90 (Thy-1)-Fluorescein (Leinco Prod. No.: C750)
Anti-Human CD90 (Thy-1)-R-phycoerythrin (Leinco Prod. No.: C751)
Anti-Mouse CD90 (Thy 1.2)-Allophycocyanin (Leinco Prod. No.: C357)
PN:
Anti-Mouse CD90 (Thy 1) (Activating)-Biotin (Leinco Prod. No.: C280)
Anti-Mouse CD90 (Thy 1) (Activating)-R-phycoerythrin (Leinco Prod. No.: C282)
Anti-Mouse CD90 (Thy 1.2)-Fluorescein (Leinco Prod. No.: C285)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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