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Anti-Human Cytokeratin 18,6 (KRT18)
Purified
Monoclonal Antibody
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Size


Prod. No.:C161
Clone:LP34
Isotype:Mouse IgG1
Conc.:0.5 mg/ml
Pkg. Size:200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human Cytokeratin 18,6 (Clone LP34) recognizes Human Cytokeratin 18,6. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Anti-Human Cytokeratin 18, 6 (clone LP34) reacts with the cytokeratin polypeptides 18 and 6, with molecular weights of 45 kDa and 56 kDa, respectively. The antibody does not react with keratins 1, 8, or 19. Anti-Cytokeratin 18, 6 reacts in cryostat sections with epithelial tissues known to contain filamentous keratins. It reacts with single glandular epithelia to stratified squamous epithelia. Anti-Cytokeratin 18,6 may be used in research studies to identify tumors as being of epithelial or mesothelial origin enabling aplastic carcinomas to be distinguished from other non-carcinoma types of tumors.1,2 The antibody may also be used to trace areas of epithelial tissue in mixed tumors.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Detergent-insoluble fraction of psoriatic human epidermis
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), IHC (Frozen)
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 10 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Moll, W. R. et al. (1982) Cell 31:11
2. Gatter et al. (1982) J. Clin. Path. 35:125
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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