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Recombinant Mouse PDGF-BB (PDGF-BB)
Purified No Carrier Protein
(Glioma-derived growth factor [GDGF], Osteosarcoma-derived Growth Factor [ODGF])
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Prod. No.:P243
SourceE. coli
Pkg. Size:2 µg, 10 µg, 100 µg, 1 mg
Storage:-20°C Detailed storage instructions below.
Description
Background:
Platelet-derived growth factor-BB homodimers (PDGF-BB) activates all combinations of PDGF receptor subunits, whereas PDGF-AA acts specifically on PDGFR alpha.1 This constitutes a positive feed-back mechanism, which, for example, could serve to potentiate autocrine stimulation of growth.2 PDGF-BB plays an important role in the cellular metabolism of vascular wall by regulating the rate of macrophage-colony stimulating factor (M-CSF) production in vascular smooth muscle cells.3 PDGF-BB is also a potent wound-healing hormones that accelerate incisional repair.4 PDGF-BB is associated with excessive cell migration, proliferation and many growth-related diseases.5 It may be a new prognostic indicator for patients with colorectal cancer.6 PDGF-BB may also induce coronary atherosclerotic disease formation by stimulating coronary artery smooth muscle cell (CASMC) migration and proliferation through agent-specific regulation of oxidative status and utilization of different signal transduction pathways.5

Source
E. coli
Molecular Weight
The predicted molecular weight of the disulfide linked homodimer Recombinant Mouse PDGF-BB is Mr 24.6 kDa.
State of Matter
Lyophilized
Formulation
This protein was 0.2 µm filtered and lyophilized from Sodium Citrate, pH 3.0.
Purity
>98% by SDS Page and HPLC
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Biological Activity
Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT


Country of Origin
USA
References
1. Josef Pfeilschifter et al. (2005) Am Society of Nephrol. 10: 1681
2. Claesson-Welsh L. et al. (1991) J Biol Chem. 266: 21138
3. Yamada N et al. (1992) J Biol Chem. 267: 15455
4. Mustoe TA et al. (1991) J Lab Clin Med. 117: 373
5. Bayraktutan U. et al. (2008) J Mol Cell Cardiol. 45: 198
6. Mori M et al. (2008) Ann Surg Oncol. 15: 2129
Related Products
Recombinant Human PDGF-BB (Leinco Prod. No.: P126)
Recombinant Rat PDGF-BB (Leinco Prod. No.: P256)
Human PDGF-BB ELISA Development Kit (Leinco Prod. No.: P134)
Anti-Human PDGF-BB (Antigen Aff. Pur.) (Leinco Prod. No.: P122)
Anti-Human PDGF-BB-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: P130)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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