Anti-Mouse CD4 (Clone GK1.5) – DyLight® 488

Anti-Mouse CD4 (Clone GK1.5) – DyLight® 488

Product No.: C1636

[product_table name="All Top" skus="C1636"]

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Clone
GK1.5
Target
CD4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD4mut, L3T4, Ly-4, Cd4, CD4 Antigen
Isotype
IgG2b
Applications
FC
,
ICC
,
IHC FF

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Product Size

Data

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (493 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this GK1.5 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IHC-FF
ICC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Background
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.1

Antigen Details

Ligand/Receptor
MHC class II molecule
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Hendrickson WA et al. (1994) Structure 2: 59
Flow Cytometry
ICC
IHC FF
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.