Anti-Mouse CD4 (Clone GK1.5) – FITC

Pricing & Details

Product No.C213
Clone
GK1.5
Protein
CD4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD4mut, L3T4, Ly-4, Cd4, CD4 Antigen
Isotype
Rat
IgG2b
Applications
FC
,
ICC
,
IHC FF
Prod No.
Size
Price
Avail.
Qty
Add to cart
C213-100 µg
100 µg
$50.00
In stock
Max:
Min: 1
Step: 1
C213-500 µg
500 µg
$90.00
In stock
Max:
Min: 1
Step: 1

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Product Concentration
0.2 mg/ml
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Excitation Laser
Blue Laser (490 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone GK1.5 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
IHC-FF
ICC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Background
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.1

Antigen Details

Protein
Ligand/Receptor
MHC class II molecule
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Hendrickson WA et al. (1994) Structure 2: 59
Flow Cytometry
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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