Anti-Mouse CD8a (Ly 2) – Purified in vivo GOLD™ Functional Grade

Mouse thymus or spleen

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

CyTOF®
CODEX®
IHC (Frozen)
IHC (Paraffin) Clone 53-6.7 has been reported for use in zinc-fixed paraffin-embedded sections and is NOT recommended for immunohistochemistry of formalin-fixed paraffin sections.
IP
B
Depletion

Clone 53-6.7 recognizes Lyt 2. Clone 53-6.7 competes with clone 5H10-1 for binding to thymocytes.

Lyt 2 is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells.

CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.

MHC class I molecule

Co-receptor for TCR

1.) Sarmiento, M. et al. (1980) Journal of Immunology 125(6):2665
2.) Gubin, M. et al. (2018) Cell. 175(4):1014–1030 Journal Link
3.) Sharma S. et al. (2020) Human Vaccines & Immunotherapeutics 16(9):2196-2203 Journal Link