Streptavidin – PE, Premium Grade

Pricing & Details

  
Prod No.
Size
Price
Avail.
Qty
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S211-0.5 mg
0.5 mg
$150.00
In stock
  
Host Species
Streptomyces avidinii
Product Concentration
0.5 mg/ml
Formulation
Streptavidin-PE is supplied in 0.01M phosphate buffered saline (PBS) pH 7.6, containing 15 mg/ml BSA and 0.05% sodium azide as a preservative.
Storage and Handling
This PE conjugate is stable when stored at 2-8°C. Do not freeze.
Working Concentration
Every lot of fluorochrome-conjugated Streptavidin-PE has been tested by flow cytometric analysis to detect biotinylated primary antibodies. From this testing it is suggested that between 0.05 and 0.2 µg of streptavidin be used per 1 x 106 cells in a 100 µl staining volume.
Isoelectric Point
pI = 5 - 6
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Description

Background
Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10 -15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.

References & Citations

1. Rowland-Jones, S. et al. (1999) Blood. 93(5):1506-10. Article Link
  
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.