Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10 -15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Every lot of fluorochrome-conjugated Streptavidin-PE has been tested by flow cytometric analysis to detect biotinylated primary antibodies. From this testing it is suggested that between 0.05 and 0.2 µg of streptavidin be used per 1 x 106 cells in a 100 µl staining volume.
Storage and Stability
This PE conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day Ambient
References & Citations
1. Rowland-Jones, S. et al. (1999) Blood. 93(5):1506-10. Article Link
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.