Streptavidin – PE, Premium Grade

Pricing & Details

Product No.S211
Formats AvailableView All
Product Type
Avidin - Biotin
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S211-0.5 mg
0.5 mg
$175.00
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Background

Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10 -15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.

Protein Details

Format
R-phycoerythrin (PE)
Product Concentration
0.2 mg/ml
Expression Host
Streptomyces avidinii
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Working Concentration
Every lot of fluorochrome-conjugated Streptavidin-PE has been tested by flow cytometric analysis to detect biotinylated primary antibodies. From this testing it is suggested that between 0.05 and 0.2 µg of streptavidin be used per 1 x 106 cells in a 100 µl staining volume.
Storage and Stability
This PE conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient

References & Citations

1. Rowland-Jones, S. et al. (1999) Blood. 93(5):1506-10. Article Link
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
S203
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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