Streptavidin – PE, Premium Grade

Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (K_d~10 ^-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Every lot of fluorochrome-conjugated Streptavidin-PE has been tested by flow cytometric analysis to detect biotinylated primary antibodies. From this testing it is suggested that between 0.05 and 0.2 µg of streptavidin be used per 1 x 10⁶ cells in a 100 µl staining volume.

This PE conjugate is stable when stored at 2-8°C. Do not freeze.

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1. Rowland-Jones, S. et al. (1999) Blood. 93(5):1506-10. Article Link