Anti-Mouse CD32/CD16 – Purified (Fc Block Antibody)

Pricing & Details

Product No.C247
Clone
2.4G2
Protein
CD32/CD16
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Fcγ R III/II, Ly-17
Isotype
Rat
IgG2b
Applications
B
,
CODEX®
,
FC
,
IHC FF
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C247-0.5mg
0.5 mg
$155.00
In stock
Max:
Min: 1
Step: 1
C247-1.0 mg
1.0 mg
$250.00
In stock
Max:
Min: 1
Step: 1

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Sorted pre-B cells
Product Concentration
0.5 mg/ml
Endotoxin Level
≤ 0.5 EU/mg as determined by the LAL method
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
Leinco antibodies are manufactured in an animal-free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 2.4G2 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this 2.4G2 antibody for use in western blotting is 1-10 μg/ml.
Additional Reported Applications For Relevant Conjugates ?
IP
B Rat Anti-Mouse CD32/CD16 Purified Fc UltraBlock™ may be used for blocking Fc receptor-mediated binding by directly labeled antibodies to reduce background staining in flow cytometry.
1. Incubate cells with Anti-Mouse CD32/CD16 Purified Fc UltraBlock™ (≤1 µg per 1 x 106 cells in 100 µl total staining volume) at 4°C for 5 minutes.
2. Add the conjugated primary antibody directly to the preincubated cells. Anti-Mouse CD32/CD16 Purified Fc UltraBlock™ does not need to be washed away prior to cell staining.For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 2.4G2 recognizes the FcγIII and FcγII receptors.
Antigen Distribution
These receptors are present on B cells, monocyte/macrophages, NK cells, neutrophils, mast cells and dendritic cells.
Background
CD16 is expressed in two forms: CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (FcγRIIIB) is a 48 kD GPI-anchored protein whose extracellular domain is over 95% homologous to that of CD16a. CD16 regulates both phagocytosis and antibody-dependent cell-mediated cytotoxicity. It has been reported that CD16 is involved in Natural Killer Cell activation and plays a role in signal transduction. The receptors, CD32 (FcγRIII) and CD16 (FcγRII), are 40-60 kD and bind antibody-antigen immune complexes and mediate adaptive immune responses.

Antigen Details

Protein
Ligand/Receptor
IgG
Function
Low affinity receptors for IgG
PubMed
NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity

References & Citations

1.) Titas, J. A. et al. (1982) J. Immunol. 133:556
2.) Rodewald, H. et al. (1992) Cell 69:139
3.) Skyberg, J. A. et al. (2020) Infection and Immunity. 88: 5 Journal Link
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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